ABSTRACT
<p><b>OBJECTIVE</b>To investigate the chemical constituents of Patrinia villosa.</p><p><b>METHOD</b>The chemical constituents were isolated by silica gel column chromatography and semi-preparative high-performance liquid chromatography, and identified by physicochemical properties and spectral analysis (MS, 1H-NMR and 13C-NMR).</p><p><b>RESULT</b>Seven compounds were isolated from ethyl acetate and n-butanol extract and identified as: 5-hydroxyl-7, 3', 4'-trimethoxy flavone (I), 5-hydroxyl-7, 4'-dimethoxy flavone (II), luteolin (III), quercetin (IV), isoorientin (V), isovitexin (VI) and 8-C glucosylprunetin (VII).</p><p><b>CONCLUSION</b>Compounds I , II, III, V, VI and VIII were obtained from the plant of genus Patrinia for the first time, compound IV was separated from P. villosa for the first time.</p>
Subject(s)
Apigenin , Chemistry , Luteolin , Chemistry , Patrinia , Chemistry , Plants, Medicinal , Chemistry , Quercetin , ChemistryABSTRACT
<p><b>AIM</b>To study the chemical constituents of Patrinia villosa Juss.</p><p><b>METHODS</b>Solvent extraction, silica gel column and preparative liquid chromatography were used to separate the chemical constituents, and the chemical structures were elucidated by physico-chemical properties and spectra data.</p><p><b>RESULTS</b>Eight compounds were isolated and identified as bolusanthol B (1), (2S)-5, 7, 2', 6'-tetrahydroxy-6,8-di (gamma,gamma-dimethylallyl) flavanone (2), orotinin (3), (2S)-5, 7, 2', 6'-tetrahydroxy-6-lavandulylated flavanone (4), 3'-prenyl-apigenine (5), luteolin (6), quercetin (7) and apigenin (8).</p><p><b>CONCLUSION</b>Compound 2 and 4 are new compounds, compounds 1, 3 and 5 were separated from Patrinia genius for the first time, compounds 6, 7 and 8 were isolated from Patrinia vollosa Juss for the first time.</p>
Subject(s)
Apigenin , Chemistry , Flavanones , Chemistry , Isoflavones , Chemistry , Luteolin , Chemistry , Molecular Conformation , Molecular Structure , Patrinia , Chemistry , Plants, Medicinal , Chemistry , Quercetin , ChemistryABSTRACT
<p><b>AIM</b>To investigate the stereoselective pharmacokinetic process of tetrahydropalmatine (THP) in rats.</p><p><b>METHODS</b>The concentrations of tetrahydropalmatine enantiomers in rat plasma were determined by coupled achiral and chiral HPLC method. The differences in plasma concentrations and pharmacokinetic parameters between the two enantiomers were compared by paired t-test.</p><p><b>RESULTS</b>The plasma levels of l-THP were always higher than those of d-THP in eight rats. There was significant difference between the main pharmacokinetic parameters of the two enantiomers.</p><p><b>CONCLUSION</b>Tetrahydropalmatine showed significant stereoselective pharmacokinetics in rats after an ig dose of the racemate.</p>
Subject(s)
Animals , Female , Male , Rats , Area Under Curve , Berberine Alkaloids , Chemistry , Pharmacokinetics , Chromatography, High Pressure Liquid , Methods , Corydalis , Chemistry , Molecular Structure , Plants, Medicinal , Chemistry , Rats, Sprague-Dawley , StereoisomerismABSTRACT
<p><b>AIM</b>To study the chemical constituents of Marsdenia tenacissima (Roxb.) Wight et Arn.</p><p><b>METHODS</b>To separate compounds with various chromatography technology and to elucidate their structures by chemical and spectral analysis.</p><p><b>RESULTS</b>Two compounds were isolated from Marsdenia tenacissima and their stuctures were determined as tenacissosides J (I) and tenacissosides K (II).</p><p><b>CONCLUSION</b>Compounds I and II are new C21 steroidal glycosides.</p>
Subject(s)
Glycosides , Chemistry , Marsdenia , Chemistry , Molecular Conformation , Molecular Structure , Plant Stems , Chemistry , Plants, Medicinal , Chemistry , Steroids , ChemistryABSTRACT
<p><b>OBJECTIVE</b>The chemical components of essential oil from fruit of Eucalyptus globulus were analyzed by GC-MS.</p><p><b>METHOD</b>The essential oil were extracted by steam distillation, then separated by capillary gas chromatography. The amount of the component from essential oil were determined by normalization methods. Chromatographic conditions were: capillary column DB-WAX (0.32 mm x 30 m, 0.25 microm) was used, column temperature: initial temperature at 40 degrees C for 3 min,ramping 5 C x min(-1) to 250 degrees C (holding for 10 min) the detector MS.</p><p><b>RESULT</b>31 component from the fruit of E. globulus were identified, which accounted for over 93.7% of total volatile oil.</p><p><b>CONCLUSION</b>The methods is reliable, stabilize and can be applied to identify the volatile oil from the fruit of E. globulus.</p>
Subject(s)
Cyclohexanols , Eucalyptus , Chemistry , Fruit , Chemistry , Gas Chromatography-Mass Spectrometry , Monoterpenes , Oils, Volatile , Chemistry , Plants, Medicinal , Chemistry , Sesquiterpenes , TerpenesABSTRACT
<p><b>OBJECTIVE</b>AIM To study the chemical constituents of the fruit of Eucalyptus globulus.</p><p><b>METHOD</b>Various column chromatographies with silica gel were employed for the isolation and purification. The structures of the compounds were elucidated with of spectral analyses and chemical methods.</p><p><b>RESULT</b>Five compounds were isolated and elucidated as follows: betulonic acid (I), betulinic acid (II), ursolic acid (III), corosolic acid (IV), daucosterol (V).</p><p><b>CONCLUSION</b>Compound I, II, III and IV were isolated from this plant for the first time.</p>
Subject(s)
Eucalyptus , Chemistry , Fruit , Chemistry , Oleanolic Acid , Chemistry , Plants, Medicinal , Chemistry , Triterpenes , ChemistryABSTRACT
<p><b>AIM</b>To study the sodium nitrite induced DNA mutation by convolution spectrometry (CS).</p><p><b>METHODS</b>The spectra of sodium nitrite-induced mutative calf thymus DNA was compared with ego criteria based on Spectra of the primary DNA within the wavelength range from 200 to 340 nm. Distilled water served as blank and normal saline served as negative control. Any difference was quantitatively expressed by differential value (delta) of convolution spectra. Near-infrared spectroscopy was employed as the reference method.</p><p><b>RESULTS</b>The differential value was positively correlated with the increasing time and concentration of sodium nitrite. delta values increased to 1.37%, 2.41% and 5.44% respectively within 2-hour's reaction between calf thymus DNA and 0.5, 0.05 and 0.005 microgram.mL-1 sodium nitrite, while on the contrary, changes could be hardly observed on the corresponding UV absorption spectra. The results were also confirmed by their corresponding near-infrared spectra.</p><p><b>CONCLUSION</b>The delta values can be used to represent the compound's strength of mutagenesis. Every convolution procedure takes less than one minute, so CS provides a fast, simple and inexpensive alternative method to determine chemical or medicinal DNA mutation.</p>
Subject(s)
Animals , Cattle , DNA , Chemistry , In Vitro Techniques , Mutation , Sodium Nitrite , Pharmacology , Spectrum Analysis , Methods , Thymus Gland , ChemistryABSTRACT
<p><b>AIM</b>To establish a suitable condition for extraction of phenylalanine (Phe), 5-hydroxytryptophan (5-OH-Trp) and four diastereomeric salts, (1R,2S)-ephedrine- (2S,3S)-tartaric acid, (1R,2S)-ephedrine-(2R,3R)-tartaric acid, (1S,2S)-pseudoephedrine-(2S,3S)-tartaric acid, (1S,2S)-pseudoephedrine- (2R,3R)-tartaric acid in supercritical fluid extraction and to assess the solubilities of Phe, 5-OH-Trp and the four diastereomeric salts in CO2.</p><p><b>METHODS</b>Single-pass method and HPCE.</p><p><b>RESULTS</b>The solubilities of Phe, 5-OH-Trp and the four diastereomeric salts in CO2 were determined over temperature and pressure ranges of 25-50 degrees C and 6.32-34.03 MPa respectively. The experimental results showed that the solubilities of Phe, 5-OH-Trp and the four diastereomeric salts do not increase with density of CO2. There existed a maximum in the critical region of CO2.</p><p><b>CONCLUSION</b>The dramatically high solubilities in the pressure of 6.32-7.78 MPa show a critical behavior, which can be explained by critical characteristic through thermodynamics analysis. The results suggest that the separation of Phe, 5-OH-Trp and the four diastereomeric salts is more efficient in critical region of CO2.</p>
Subject(s)
5-Hydroxytryptophan , Chemistry , Ephedrine , Chemistry , Phenylalanine , Chemistry , Pressure , Solubility , Stereoisomerism , Tartrates , Chemistry , TemperatureABSTRACT
Objective：To establish a capillary gas chromatography(CGC) method for identifying the origin fungi of traditional Chinese medicine Hongqu. Methods：The volatile components of 7 species of Monascus including M. purpureus Went, M.aurantiacus Lee, M. serorubesceus Sato, M. albidus Sato, M. barkeri Dangerd, M. ruber van Tieghem, and M.fuliginosus Sato were analyzed by capillary gas chromatography method. Inlet temperature and detector temperature were 280℃，temperature program were 130℃,5 min→10℃/min→200℃, 10 min FID was used for detection. Results：The variety and contents of the volatility components in Monascus had obvious differences which can be distinguished easily by the main fingerprint peaks within 20 min. Conclusion：This method is useful to identify of Monascus fungi.
ABSTRACT
Objective：To establish a capillary gas chromatography(CGC) method for identifying the origin fungi of traditional Chinese medicine Hongqu. Methods：The volatile components of 7 species of Monascus including M. purpureus Went, M.aurantiacus Lee, M. serorubesceus Sato, M. albidus Sato, M. barkeri Dangerd, M. ruber van Tieghem, and M.fuliginosus Sato were analyzed by capillary gas chromatography method. Inlet temperature and detector temperature were 280℃，temperature program were 130℃,5 min→10℃/min→200℃, 10 min FID was used for detection. Results：The variety and contents of the volatility components in Monascus had obvious differences which can be distinguished easily by the main fingerprint peaks within 20 min. Conclusion：This method is useful to identify of Monascus fungi.